Adaptación en el intestino corto: efecto de la nutrición enteral mínima y los probióticos sobre la proliferación y la apoptosis en la pared intestinal.

I. Eizaguirre; N. García Urkia; A. B. Asensio; E. Hijona; J. M. García Arenzana; P. Bachiller; P. Aldazabal

Adaptación en el intestino corto: efecto de la nutrición enteral mínima y los probióticos sobre la proliferación y la apoptosis en la pared intestinal.

Translated title of the contribution: [Adaptation in the small intestine: Effect of minimal enteral nutrition and probiotics on proliferation and apoptosis in the intestinal wall].

I. Eizaguirre, N. García Urkia, A. B. Asensio, E. Hijona, J. M. García Arenzana, P. Bachiller, P. Aldazabal

Biomaterials

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The intestinal wall integrity is central to the barrier function and depends on the balance of proliferation/apoptosis. Short bowel (SB) or Parenteral Nutrition (PN) induce high bacterial translocation (BT) probably by the intestinal barrier bug. Probiotics or minimal enteral nutrition (MEN) have reduced BT in animal models. Determine in two BT animal models (SB or PN) the effect of MEN or probiotics on proliferation and apoptosis rates of the intestinal wall. Seventy-one Wistar rats, divided into 4 groups: 1) PN (N = 23): parenteral nutrition; 2) PNMEN (N = 16): PN + MEN (2.9 g/100 g/day standard diet); 3) RES (N = 15): 80% bowel resection and standard oral diet; 4) RESPROB (N = 17): RES + probiotics (7 X 10(9) CFU Bifidobacterium lactis). After 10 days in metabolic cages, mesenteryc lymph nodes, portal blood and peripheral blood were cultured. By immunohistochemistry, proliferation and apoptosis index were calculated as well as the proliferation-apoptosis rate. BT: decreased in PNMEN (45%) and RESPROB groups (35%) versus PN (65%) and RES (67%) groups (p<0.05). Proliferation index: was better in PNMEN (12,07) and RESPROB (13,93) groups than PN (7,45) and RES (5,54) groups. (p</0.05). Apoptosis index: PNMEN group had 7,81 and PN group 14,90. (p<0,05). Proliferation-apoptosis rate: was higher in PNMEN (1,54) and RESPROB (1,67) groups than PN (0,50) ans RES (0,71) groups. (p<0.01). MEN and probiotics reduce BT and improve cellular renewal by encouraging the proliferation. MEN also prevents apoptosis.

Translated title of the contribution	[Adaptation in the small intestine: Effect of minimal enteral nutrition and probiotics on proliferation and apoptosis in the intestinal wall].
Original language	Spanish
Pages (from-to)	118-121
Number of pages	4
Journal	Cirugia pediatrica : organo oficial de la Sociedad Espanola de Cirugia Pediatrica
Volume	23
Issue number	2
Publication status	Published - Apr 2010

Cite this

Eizaguirre, I., García Urkia, N., Asensio, A. B., Hijona, E., García Arenzana, J. M., Bachiller, P., & Aldazabal, P. (2010). Adaptación en el intestino corto: efecto de la nutrición enteral mínima y los probióticos sobre la proliferación y la apoptosis en la pared intestinal. Cirugia pediatrica : organo oficial de la Sociedad Espanola de Cirugia Pediatrica, 23(2), 118-121.

@article{f7fdbfeae7a44e5685a1bf470802647b,

title = "Adaptaci{\'o}n en el intestino corto: efecto de la nutrici{\'o}n enteral m{\'i}nima y los probi{\'o}ticos sobre la proliferaci{\'o}n y la apoptosis en la pared intestinal.",

abstract = "The intestinal wall integrity is central to the barrier function and depends on the balance of proliferation/apoptosis. Short bowel (SB) or Parenteral Nutrition (PN) induce high bacterial translocation (BT) probably by the intestinal barrier bug. Probiotics or minimal enteral nutrition (MEN) have reduced BT in animal models. Determine in two BT animal models (SB or PN) the effect of MEN or probiotics on proliferation and apoptosis rates of the intestinal wall. Seventy-one Wistar rats, divided into 4 groups: 1) PN (N = 23): parenteral nutrition; 2) PNMEN (N = 16): PN + MEN (2.9 g/100 g/day standard diet); 3) RES (N = 15): 80% bowel resection and standard oral diet; 4) RESPROB (N = 17): RES + probiotics (7 X 10(9) CFU Bifidobacterium lactis). After 10 days in metabolic cages, mesenteryc lymph nodes, portal blood and peripheral blood were cultured. By immunohistochemistry, proliferation and apoptosis index were calculated as well as the proliferation-apoptosis rate. BT: decreased in PNMEN (45%) and RESPROB groups (35%) versus PN (65%) and RES (67%) groups (p<0.05). Proliferation index: was better in PNMEN (12,07) and RESPROB (13,93) groups than PN (7,45) and RES (5,54) groups. (p</0.05). Apoptosis index: PNMEN group had 7,81 and PN group 14,90. (p<0,05). Proliferation-apoptosis rate: was higher in PNMEN (1,54) and RESPROB (1,67) groups than PN (0,50) ans RES (0,71) groups. (p<0.01). MEN and probiotics reduce BT and improve cellular renewal by encouraging the proliferation. MEN also prevents apoptosis.",

author = "I. Eizaguirre and {Garc{\'i}a Urkia}, N. and Asensio, {A. B.} and E. Hijona and {Garc{\'i}a Arenzana}, {J. M.} and P. Bachiller and P. Aldazabal",

year = "2010",

month = apr,

language = "Espa{\~n}ol",

volume = "23",

pages = "118--121",

journal = "Cirugia pediatrica : organo oficial de la Sociedad Espanola de Cirugia Pediatrica",

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Eizaguirre, I, García Urkia, N, Asensio, AB, Hijona, E, García Arenzana, JM, Bachiller, P & Aldazabal, P 2010, 'Adaptación en el intestino corto: efecto de la nutrición enteral mínima y los probióticos sobre la proliferación y la apoptosis en la pared intestinal.', Cirugia pediatrica : organo oficial de la Sociedad Espanola de Cirugia Pediatrica, vol. 23, no. 2, pp. 118-121.

Adaptación en el intestino corto: efecto de la nutrición enteral mínima y los probióticos sobre la proliferación y la apoptosis en la pared intestinal. / Eizaguirre, I.; García Urkia, N.; Asensio, A. B. et al.
In: Cirugia pediatrica : organo oficial de la Sociedad Espanola de Cirugia Pediatrica, Vol. 23, No. 2, 04.2010, p. 118-121.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Adaptación en el intestino corto

T2 - efecto de la nutrición enteral mínima y los probióticos sobre la proliferación y la apoptosis en la pared intestinal.

AU - Eizaguirre, I.

AU - García Urkia, N.

AU - Asensio, A. B.

AU - Hijona, E.

AU - García Arenzana, J. M.

AU - Bachiller, P.

AU - Aldazabal, P.

PY - 2010/4

Y1 - 2010/4

N2 - The intestinal wall integrity is central to the barrier function and depends on the balance of proliferation/apoptosis. Short bowel (SB) or Parenteral Nutrition (PN) induce high bacterial translocation (BT) probably by the intestinal barrier bug. Probiotics or minimal enteral nutrition (MEN) have reduced BT in animal models. Determine in two BT animal models (SB or PN) the effect of MEN or probiotics on proliferation and apoptosis rates of the intestinal wall. Seventy-one Wistar rats, divided into 4 groups: 1) PN (N = 23): parenteral nutrition; 2) PNMEN (N = 16): PN + MEN (2.9 g/100 g/day standard diet); 3) RES (N = 15): 80% bowel resection and standard oral diet; 4) RESPROB (N = 17): RES + probiotics (7 X 10(9) CFU Bifidobacterium lactis). After 10 days in metabolic cages, mesenteryc lymph nodes, portal blood and peripheral blood were cultured. By immunohistochemistry, proliferation and apoptosis index were calculated as well as the proliferation-apoptosis rate. BT: decreased in PNMEN (45%) and RESPROB groups (35%) versus PN (65%) and RES (67%) groups (p<0.05). Proliferation index: was better in PNMEN (12,07) and RESPROB (13,93) groups than PN (7,45) and RES (5,54) groups. (p</0.05). Apoptosis index: PNMEN group had 7,81 and PN group 14,90. (p<0,05). Proliferation-apoptosis rate: was higher in PNMEN (1,54) and RESPROB (1,67) groups than PN (0,50) ans RES (0,71) groups. (p<0.01). MEN and probiotics reduce BT and improve cellular renewal by encouraging the proliferation. MEN also prevents apoptosis.

AB - The intestinal wall integrity is central to the barrier function and depends on the balance of proliferation/apoptosis. Short bowel (SB) or Parenteral Nutrition (PN) induce high bacterial translocation (BT) probably by the intestinal barrier bug. Probiotics or minimal enteral nutrition (MEN) have reduced BT in animal models. Determine in two BT animal models (SB or PN) the effect of MEN or probiotics on proliferation and apoptosis rates of the intestinal wall. Seventy-one Wistar rats, divided into 4 groups: 1) PN (N = 23): parenteral nutrition; 2) PNMEN (N = 16): PN + MEN (2.9 g/100 g/day standard diet); 3) RES (N = 15): 80% bowel resection and standard oral diet; 4) RESPROB (N = 17): RES + probiotics (7 X 10(9) CFU Bifidobacterium lactis). After 10 days in metabolic cages, mesenteryc lymph nodes, portal blood and peripheral blood were cultured. By immunohistochemistry, proliferation and apoptosis index were calculated as well as the proliferation-apoptosis rate. BT: decreased in PNMEN (45%) and RESPROB groups (35%) versus PN (65%) and RES (67%) groups (p<0.05). Proliferation index: was better in PNMEN (12,07) and RESPROB (13,93) groups than PN (7,45) and RES (5,54) groups. (p</0.05). Apoptosis index: PNMEN group had 7,81 and PN group 14,90. (p<0,05). Proliferation-apoptosis rate: was higher in PNMEN (1,54) and RESPROB (1,67) groups than PN (0,50) ans RES (0,71) groups. (p<0.01). MEN and probiotics reduce BT and improve cellular renewal by encouraging the proliferation. MEN also prevents apoptosis.

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M3 - Artículo

C2 - 21298924

AN - SCOPUS:79952261283

SN - 0214-1221

VL - 23

SP - 118

EP - 121

JO - Cirugia pediatrica : organo oficial de la Sociedad Espanola de Cirugia Pediatrica

JF - Cirugia pediatrica : organo oficial de la Sociedad Espanola de Cirugia Pediatrica

IS - 2

ER -

Adaptación en el intestino corto: efecto de la nutrición enteral mínima y los probióticos sobre la proliferación y la apoptosis en la pared intestinal.

Abstract

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