TY - GEN
T1 - Automatic segmentation of embryonic heart in time-lapse fluorescence microscopy image sequences
AU - Krämer, P.
AU - Boto, F.
AU - Wald, D.
AU - Bessy, F.
AU - Paloc, C.
AU - Callol, C.
AU - Letamendia, A.
AU - Ibarbia, I.
AU - Holgado, O.
AU - Virto, J. M.
PY - 2010
Y1 - 2010
N2 - Embryos of animal models are becoming widely used to study cardiac development and genetics. However, the analysis of the embryonic heart is still mostly done manually. This is a very laborious and expensive task as each embryo has to be inspected visually by a biologist. We therefore propose to automatically segment the embryonic heart from high-speed fluorescence microscopy image sequences, allowing morphological and functional quantitative features of cardiac activity to be extracted. Several methods are presented and compared within a large range of images, varying in quality, acquisition parameters, and embryos position. Although manual control and visual assessment would still be necessary, the best of our methods has the potential to drastically reduce biologist workload by automating manual segmentation.
AB - Embryos of animal models are becoming widely used to study cardiac development and genetics. However, the analysis of the embryonic heart is still mostly done manually. This is a very laborious and expensive task as each embryo has to be inspected visually by a biologist. We therefore propose to automatically segment the embryonic heart from high-speed fluorescence microscopy image sequences, allowing morphological and functional quantitative features of cardiac activity to be extracted. Several methods are presented and compared within a large range of images, varying in quality, acquisition parameters, and embryos position. Although manual control and visual assessment would still be necessary, the best of our methods has the potential to drastically reduce biologist workload by automating manual segmentation.
KW - Embryonic heart
KW - Fluorescent microscopy images
KW - Segmentation
UR - https://www.scopus.com/pages/publications/77956445594
M3 - Conference contribution
AN - SCOPUS:77956445594
SN - 9789896740184
T3 - BIOSIGNALS 2010 - Proceedings of the 3rd International Conference on Bio-inpsired Systems and Signal Processing, Proceedings
SP - 121
EP - 126
BT - BIOSIGNALS 2010 - Proceedings of the 3rd International Conference on Bio-inpsired Systems and Signal Processing
T2 - 3rd International Conference on Bio-inspired Systems and Signal Processing, BIOSIGNALS 2010
Y2 - 20 January 2010 through 23 January 2010
ER -