Biorreactor de reflexión interna múltiple para la detección in situ de peróxido de hidrógeno

Bergoi Ibarlucea; Jordi Vila-Planas; Víctor Javier Cadarso; Jahir Orozco; Stefanie Demming; César Fernández-Sánchez; Ralph Wilke; Stephanus Büttgenbach; Carlos Domínguez; Andreu Llobera

Biorreactor de reflexión interna múltiple para la detección in situ de peróxido de hidrógeno

Translated title of the contribution: Multiple internal reflection bioreactor for in situ detection of hydrogen peroxide

Bergoi Ibarlucea^*
, Jordi Vila-Planas
, Víctor Javier Cadarso
, Jahir Orozco
, Stefanie Demming
, César Fernández-Sánchez
, Ralph Wilke
, Stephanus Büttgenbach
, Carlos Domínguez
, Andreu Llobera

^*Corresponding author for this work

Technical University of Braunschweig

Research output: Contribution to journal › Article › peer-review

Abstract

Taking advantage of the poly(dimethyl siloxane) (PDMS) optical and structural properties, it is possible to develop a highly sensitive PDMS-based photonic lab on a chip, which has been named Propagating Multiple Internal Reflection (PMIR) system. For the application in this system, the surface of PDMS was chemically modified and then Horseradish Peroxidase (HRP) was immobilized, having then a bioreactor with photonic transduction. Absorbance measurements in 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) ABTS solutions with increasing hydrogen peroxide (H₂O₂) concentrations allowed to determine the peroxide with a sensitivity of 0.025±0.004 (a.u.)/μM and a limit of detection (LOD) of 2.8±0.4 μM.

Translated title of the contribution	Multiple internal reflection bioreactor for in situ detection of hydrogen peroxide
Original language	Spanish
Pages (from-to)	203-208
Number of pages	6
Journal	Optica Pura y Aplicada
Volume	42
Issue number	4
Publication status	Published - Dec 2009
Externally published	Yes

Cite this

@article{62bcd068aaa04812bc74a85c70d6f1b3,

title = "Biorreactor de reflexi{\'o}n interna m{\'u}ltiple para la detecci{\'o}n in situ de per{\'o}xido de hidr{\'o}geno",

abstract = "Taking advantage of the poly(dimethyl siloxane) (PDMS) optical and structural properties, it is possible to develop a highly sensitive PDMS-based photonic lab on a chip, which has been named Propagating Multiple Internal Reflection (PMIR) system. For the application in this system, the surface of PDMS was chemically modified and then Horseradish Peroxidase (HRP) was immobilized, having then a bioreactor with photonic transduction. Absorbance measurements in 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) ABTS solutions with increasing hydrogen peroxide (H2O2) concentrations allowed to determine the peroxide with a sensitivity of 0.025±0.004 (a.u.)/μM and a limit of detection (LOD) of 2.8±0.4 μM.",

keywords = "Biofunctionalization, Horseradish Peroxidase, Lab-on-a-Chip, PDMS, PMIR, Photonics",

author = "Bergoi Ibarlucea and Jordi Vila-Planas and Cadarso, \{V{\'i}ctor Javier\} and Jahir Orozco and Stefanie Demming and C{\'e}sar Fern{\'a}ndez-S{\'a}nchez and Ralph Wilke and Stephanus B{\"u}ttgenbach and Carlos Dom{\'i}nguez and Andreu Llobera",

year = "2009",

month = dec,

language = "Espa{\~n}ol",

volume = "42",

pages = "203--208",

journal = "Optica Pura y Aplicada",

issn = "0030-3917",

publisher = "Sociedad Espanola de Optica",

number = "4",

}

TY - JOUR

T1 - Biorreactor de reflexión interna múltiple para la detección in situ de peróxido de hidrógeno

AU - Ibarlucea, Bergoi

AU - Vila-Planas, Jordi

AU - Cadarso, Víctor Javier

AU - Orozco, Jahir

AU - Demming, Stefanie

AU - Fernández-Sánchez, César

AU - Wilke, Ralph

AU - Büttgenbach, Stephanus

AU - Domínguez, Carlos

AU - Llobera, Andreu

PY - 2009/12

Y1 - 2009/12

N2 - Taking advantage of the poly(dimethyl siloxane) (PDMS) optical and structural properties, it is possible to develop a highly sensitive PDMS-based photonic lab on a chip, which has been named Propagating Multiple Internal Reflection (PMIR) system. For the application in this system, the surface of PDMS was chemically modified and then Horseradish Peroxidase (HRP) was immobilized, having then a bioreactor with photonic transduction. Absorbance measurements in 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) ABTS solutions with increasing hydrogen peroxide (H2O2) concentrations allowed to determine the peroxide with a sensitivity of 0.025±0.004 (a.u.)/μM and a limit of detection (LOD) of 2.8±0.4 μM.

AB - Taking advantage of the poly(dimethyl siloxane) (PDMS) optical and structural properties, it is possible to develop a highly sensitive PDMS-based photonic lab on a chip, which has been named Propagating Multiple Internal Reflection (PMIR) system. For the application in this system, the surface of PDMS was chemically modified and then Horseradish Peroxidase (HRP) was immobilized, having then a bioreactor with photonic transduction. Absorbance measurements in 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) ABTS solutions with increasing hydrogen peroxide (H2O2) concentrations allowed to determine the peroxide with a sensitivity of 0.025±0.004 (a.u.)/μM and a limit of detection (LOD) of 2.8±0.4 μM.

KW - Biofunctionalization

KW - Horseradish Peroxidase

KW - Lab-on-a-Chip

KW - PDMS

KW - PMIR

KW - Photonics

UR - https://www.scopus.com/pages/publications/75349100914

M3 - Artículo

AN - SCOPUS:75349100914

SN - 0030-3917

VL - 42

SP - 203

EP - 208

JO - Optica Pura y Aplicada

JF - Optica Pura y Aplicada

IS - 4

ER -

Biorreactor de reflexión interna múltiple para la detección in situ de peróxido de hidrógeno

Abstract

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