C-reactive protein aptasensor for early sepsis diagnosis by means of an optical fiber device

  • C. R. Zamarreño
  • , I. Ardaiz
  • , L. Ruete
  • , F. J. Muñoz
  • , I. R. Matias
  • , F. J. Arregui

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

15 Citations (Scopus)

Abstract

This work presents the development of a fast response, low cost and real time monitoring biosensor for sepsis diagnosis by means of the detection of C-reactive protein (CRP) as a sepsis biomarker. The developed sensor has two main features. The first one consists of the utilization of high sensitive optical fiber refractometers based on Lossy Mode Resonances (LMRs). The second is the utilization of CRP-selective and high sensitive aptamer chains as the sensing element (bio-receptor). Thin polymer/aptamer films were fabricated using the Layer-by-Layer (LbL) technique onto the optical fiber device in order to facilitate the detection of the aptamer refractive index changes in presence of CRP. Obtained devices were able to discriminate between a non-pathological concentration (2mg/L) and a pathological concentration (20mg/L) of CRP in less than 15 min. In addition, these sensors could be reused after a proper decontamination process which enhances their lifetime.

Original languageEnglish
Title of host publicationIEEE SENSORS 2013 - Proceedings
PublisherIEEE Computer Society
ISBN (Print)9781467346405
DOIs
Publication statusPublished - 2013
Externally publishedYes
Event12th IEEE SENSORS 2013 Conference - Baltimore, MD, United States
Duration: 4 Nov 20136 Nov 2013

Publication series

NameProceedings of IEEE Sensors

Conference

Conference12th IEEE SENSORS 2013 Conference
Country/TerritoryUnited States
CityBaltimore, MD
Period4/11/136/11/13

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