Culturing of neurons in microfluidic arrays

  • L. Griscom
  • , P. Degenaar
  • , M. Denoual
  • , F. Morin

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

9 Citations (Scopus)

Abstract

The ability to culture cells on microchips, containing arrays of micro-chambers, is becoming an important diagnostic tool to the biomedical researcher. In this work different neural cell types are cultured in microfluidic arrays made from glass and PDMS substrates.. The microfluidic arrays are made using photolithographically patterned two-layer SU-8 mold and transferred to the PDMS. This allows the creation of interconnected microchambers, which permit physically isolated cells to form axons in preferential directions. The culturing of several different neural cell types was performed using PC-12, P19 and primary culture neural cells. The viability of the cells and integration with microelectrode arrays was tested.

Original languageEnglish
Title of host publication2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings
EditorsDavid Beebe, Andre Dittmar
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages160-163
Number of pages4
ISBN (Electronic)0780374800, 9780780374805
DOIs
Publication statusPublished - 2002
Externally publishedYes
Event2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Madison, United States
Duration: 2 May 20024 May 2002

Publication series

Name2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings

Conference

Conference2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology
Country/TerritoryUnited States
CityMadison
Period2/05/024/05/02

Keywords

  • Microelectrode arrays Neurons
  • Microfluidics
  • PDMS

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