Detection and differentiation of Leptospira spp. serovars in bovine semen by polymerase chain reaction and restriction fragment length polymorphism

Marcos Bryan Heinemann; José Fernando Garcia; Cáris Maroni Nunes; Fabio Gregori; Zenaide Maria Moraes Higa; Silvio Arruda Vasconcellos; Leonardo José Richtzenhain

doi:10.1016/S0378-1135(00)00150-4

Detection and differentiation of Leptospira spp. serovars in bovine semen by polymerase chain reaction and restriction fragment length polymorphism

Marcos Bryan Heinemann
, José Fernando Garcia^*
, Cáris Maroni Nunes
, Fabio Gregori
, Zenaide Maria Moraes Higa
, Silvio Arruda Vasconcellos
, Leonardo José Richtzenhain

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

In view of the importance of venereal transmission of bovine leptospirosis, the objective of the present study was to apply the polymerase chain reaction (PCR) to 26 serovars of Leptospira interrogans, L. borgpetersenii, L. santarosai, L. noguchii and L. biflexa, to determine the detection threshold in semen samples and to evaluate the possibility of differentiation among serovars using 19 restriction endonucleases. The results showed that all serovars were amplified and the detection threshold in semen samples of a bull was 100 bacteria/ml. Using endonucleases we could classify the 26 serovars into eight groups. The present results show that PCR is a method of great potential for the detection of Leptospira spp. at bovine artificial insemination centers. (C) 2000 Elsevier Science B.V.

Original language	English
Pages (from-to)	261-267
Number of pages	7
Journal	Veterinary Microbiology
Volume	73
Issue number	4
DOIs	https://doi.org/10.1016/S0378-1135(00)00150-4
Publication status	Published - 11 May 2000
Externally published	Yes

Keywords

Cattle-bacteria
Leptospira spp.
PCR
RFLP

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10.1016/S0378-1135(00)00150-4

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title = "Detection and differentiation of Leptospira spp. serovars in bovine semen by polymerase chain reaction and restriction fragment length polymorphism",

abstract = "In view of the importance of venereal transmission of bovine leptospirosis, the objective of the present study was to apply the polymerase chain reaction (PCR) to 26 serovars of Leptospira interrogans, L. borgpetersenii, L. santarosai, L. noguchii and L. biflexa, to determine the detection threshold in semen samples and to evaluate the possibility of differentiation among serovars using 19 restriction endonucleases. The results showed that all serovars were amplified and the detection threshold in semen samples of a bull was 100 bacteria/ml. Using endonucleases we could classify the 26 serovars into eight groups. The present results show that PCR is a method of great potential for the detection of Leptospira spp. at bovine artificial insemination centers. (C) 2000 Elsevier Science B.V.",

keywords = "Cattle-bacteria, Leptospira spp., PCR, RFLP",

author = "Heinemann, \{Marcos Bryan\} and Garcia, \{Jos{\'e} Fernando\} and Nunes, \{C{\'a}ris Maroni\} and Fabio Gregori and Higa, \{Zenaide Maria Moraes\} and Vasconcellos, \{Silvio Arruda\} and Richtzenhain, \{Leonardo Jos{\'e}\}",

year = "2000",

month = may,

day = "11",

doi = "10.1016/S0378-1135(00)00150-4",

language = "English",

volume = "73",

pages = "261--267",

journal = "Veterinary Microbiology",

issn = "0378-1135",

publisher = "Elsevier",

number = "4",

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TY - JOUR

T1 - Detection and differentiation of Leptospira spp. serovars in bovine semen by polymerase chain reaction and restriction fragment length polymorphism

AU - Heinemann, Marcos Bryan

AU - Garcia, José Fernando

AU - Nunes, Cáris Maroni

AU - Gregori, Fabio

AU - Higa, Zenaide Maria Moraes

AU - Vasconcellos, Silvio Arruda

AU - Richtzenhain, Leonardo José

PY - 2000/5/11

Y1 - 2000/5/11

N2 - In view of the importance of venereal transmission of bovine leptospirosis, the objective of the present study was to apply the polymerase chain reaction (PCR) to 26 serovars of Leptospira interrogans, L. borgpetersenii, L. santarosai, L. noguchii and L. biflexa, to determine the detection threshold in semen samples and to evaluate the possibility of differentiation among serovars using 19 restriction endonucleases. The results showed that all serovars were amplified and the detection threshold in semen samples of a bull was 100 bacteria/ml. Using endonucleases we could classify the 26 serovars into eight groups. The present results show that PCR is a method of great potential for the detection of Leptospira spp. at bovine artificial insemination centers. (C) 2000 Elsevier Science B.V.

AB - In view of the importance of venereal transmission of bovine leptospirosis, the objective of the present study was to apply the polymerase chain reaction (PCR) to 26 serovars of Leptospira interrogans, L. borgpetersenii, L. santarosai, L. noguchii and L. biflexa, to determine the detection threshold in semen samples and to evaluate the possibility of differentiation among serovars using 19 restriction endonucleases. The results showed that all serovars were amplified and the detection threshold in semen samples of a bull was 100 bacteria/ml. Using endonucleases we could classify the 26 serovars into eight groups. The present results show that PCR is a method of great potential for the detection of Leptospira spp. at bovine artificial insemination centers. (C) 2000 Elsevier Science B.V.

KW - Cattle-bacteria

KW - Leptospira spp.

KW - PCR

KW - RFLP

UR - https://www.scopus.com/pages/publications/0034636397

U2 - 10.1016/S0378-1135(00)00150-4

DO - 10.1016/S0378-1135(00)00150-4

M3 - Article

C2 - 10781725

AN - SCOPUS:0034636397

SN - 0378-1135

VL - 73

SP - 261

EP - 267

JO - Veterinary Microbiology

JF - Veterinary Microbiology

IS - 4

ER -

Detection and differentiation of Leptospira spp. serovars in bovine semen by polymerase chain reaction and restriction fragment length polymorphism

Abstract

Keywords

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