TY - GEN
T1 - Disposable multiple internal reflection systems for photonic cell screening
AU - Ibarlucea, B.
AU - Vila-Planas, J.
AU - Fernández-Rosas, E.
AU - Demming, S.
AU - Nogues, C.
AU - Plaza, J. A.
AU - Büttgenbach, S.
AU - Llobera, A.
PY - 2010
Y1 - 2010
N2 - A low-cost photonic lab on a chip with three different working regimes for cell screening is presented, namely scattering, scattering+absorbance and absorbance. In all regimes, the whole spectral response in absorbance is obtained, being possible to determine for each regime which is the optimal working wavelength that provides the highest performance. Experimental limits of detection (LOD) of 54.9 ± 0.7 cells, 53 ± 1 cells, and 105 ± 4 cells, depending on the working regime used, have been obtained. The system has also been used for measuring the dead/live cell ratio, obtaining LODs between 7.6 ± 0.4% and 6.7 ± 0.3% of dead cells.
AB - A low-cost photonic lab on a chip with three different working regimes for cell screening is presented, namely scattering, scattering+absorbance and absorbance. In all regimes, the whole spectral response in absorbance is obtained, being possible to determine for each regime which is the optimal working wavelength that provides the highest performance. Experimental limits of detection (LOD) of 54.9 ± 0.7 cells, 53 ± 1 cells, and 105 ± 4 cells, depending on the working regime used, have been obtained. The system has also been used for measuring the dead/live cell ratio, obtaining LODs between 7.6 ± 0.4% and 6.7 ± 0.3% of dead cells.
KW - Cell screening
KW - PDMS
KW - PMIR
KW - Photonic lab-on-a-chip
UR - https://www.scopus.com/pages/publications/84884391335
M3 - Conference contribution
AN - SCOPUS:84884391335
SN - 9781618390622
T3 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
SP - 941
EP - 943
BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
T2 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Y2 - 3 October 2010 through 7 October 2010
ER -