Generation of humanized bone for disease modeling using porcine adipose tissue-derived extracellular matrix scaffolds and human dental pulp stem cells

Iratxe Madarieta; Jon Luzuriaga; Jone Salvador-Moya; Beatriz Pardo-Rodríguez; Patricia García-Gallastegui; Nerea García-Urkia; Igor Irastorza; Francisco Javier Fernandez-San-Argimiro; Verónica Uribe-Etxebarria; Lucía Jiménez-Rojo; Fernando Unda; Beatriz Olalde; Jose Ramon Pineda; Gaskon Ibarretxe

doi:10.1016/bs.mcb.2025.06.003

Generation of humanized bone for disease modeling using porcine adipose tissue-derived extracellular matrix scaffolds and human dental pulp stem cells

Iratxe Madarieta^*, Jon Luzuriaga, Jone Salvador-Moya, Beatriz Pardo-Rodríguez, Patricia García-Gallastegui, Nerea García-Urkia, Igor Irastorza, Francisco Javier Fernandez-San-Argimiro, Verónica Uribe-Etxebarria, Lucía Jiménez-Rojo, Fernando Unda, Beatriz Olalde, Jose Ramon Pineda, Gaskon Ibarretxe

^*Corresponding author for this work

Biomaterials

Research output: Contribution to journal › Article › peer-review

Abstract

Human Dental Pulp Stem Cells (hDPSCs) represent a remarkable cell source for tissue engineering and regenerative medicine, offering significant potential for use in personalized medicine and autologous therapies. Decellularized extracellular matrix (ECM)-derived biological scaffolds show excellent properties for supporting cell delivery and growth in both in vitro and in vivo applications. These scaffolds provide essential biochemical cues that regulate cellular functions and offer a more accurate representation of the in vivo environment. Porcine decellularized adipose tissue (pDAT) is a very abundant source of ECM, constituting an ideal material for biologic scaffold preparation. The integration of hDPSCs with pDAT-derived ECM enables the patient-specific generation of diverse humanized tissues and their application in personalized drug screening platforms. This chapter details a three dimensional (3D) culture methodology utilizing hDPSCs and pDAT-derived scaffolds to engineer humanized bone tissue.

Original language	English
Journal	Methods in Cell Biology
DOIs	https://doi.org/10.1016/bs.mcb.2025.06.003
Publication status	Accepted/In press - 2025

Keywords

3D culture
Adipose tissue
Biologic scaffold
Cell differentiation
Decellularization
Extracellular matrix
Human dental pulp stem cells
Osteogenesis
Solid foam
Tissue engineering

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10.1016/bs.mcb.2025.06.003

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Madarieta, I., Luzuriaga, J., Salvador-Moya, J., Pardo-Rodríguez, B., García-Gallastegui, P., García-Urkia, N., Irastorza, I., Fernandez-San-Argimiro, F. J., Uribe-Etxebarria, V., Jiménez-Rojo, L., Unda, F., Olalde, B., Pineda, J. R., & Ibarretxe, G. (Accepted/In press). Generation of humanized bone for disease modeling using porcine adipose tissue-derived extracellular matrix scaffolds and human dental pulp stem cells. Methods in Cell Biology. https://doi.org/10.1016/bs.mcb.2025.06.003

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title = "Generation of humanized bone for disease modeling using porcine adipose tissue-derived extracellular matrix scaffolds and human dental pulp stem cells",

abstract = "Human Dental Pulp Stem Cells (hDPSCs) represent a remarkable cell source for tissue engineering and regenerative medicine, offering significant potential for use in personalized medicine and autologous therapies. Decellularized extracellular matrix (ECM)-derived biological scaffolds show excellent properties for supporting cell delivery and growth in both in vitro and in vivo applications. These scaffolds provide essential biochemical cues that regulate cellular functions and offer a more accurate representation of the in vivo environment. Porcine decellularized adipose tissue (pDAT) is a very abundant source of ECM, constituting an ideal material for biologic scaffold preparation. The integration of hDPSCs with pDAT-derived ECM enables the patient-specific generation of diverse humanized tissues and their application in personalized drug screening platforms. This chapter details a three dimensional (3D) culture methodology utilizing hDPSCs and pDAT-derived scaffolds to engineer humanized bone tissue.",

keywords = "3D culture, Adipose tissue, Biologic scaffold, Cell differentiation, Decellularization, Extracellular matrix, Human dental pulp stem cells, Osteogenesis, Solid foam, Tissue engineering",

author = "Iratxe Madarieta and Jon Luzuriaga and Jone Salvador-Moya and Beatriz Pardo-Rodr{\'i}guez and Patricia Garc{\'i}a-Gallastegui and Nerea Garc{\'i}a-Urkia and Igor Irastorza and Fernandez-San-Argimiro, \{Francisco Javier\} and Ver{\'o}nica Uribe-Etxebarria and Luc{\'i}a Jim{\'e}nez-Rojo and Fernando Unda and Beatriz Olalde and Pineda, \{Jose Ramon\} and Gaskon Ibarretxe",

note = "Publisher Copyright: {\textcopyright} 2025",

year = "2025",

doi = "10.1016/bs.mcb.2025.06.003",

language = "English",

journal = "Methods in Cell Biology",

issn = "0091-679X",

publisher = "Academic Press Inc.",

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Madarieta, I, Luzuriaga, J, Salvador-Moya, J, Pardo-Rodríguez, B, García-Gallastegui, P, García-Urkia, N, Irastorza, I, Fernandez-San-Argimiro, FJ, Uribe-Etxebarria, V, Jiménez-Rojo, L, Unda, F, Olalde, B, Pineda, JR & Ibarretxe, G 2025, 'Generation of humanized bone for disease modeling using porcine adipose tissue-derived extracellular matrix scaffolds and human dental pulp stem cells', Methods in Cell Biology. https://doi.org/10.1016/bs.mcb.2025.06.003

TY - JOUR

T1 - Generation of humanized bone for disease modeling using porcine adipose tissue-derived extracellular matrix scaffolds and human dental pulp stem cells

AU - Madarieta, Iratxe

AU - Luzuriaga, Jon

AU - Salvador-Moya, Jone

AU - Pardo-Rodríguez, Beatriz

AU - García-Gallastegui, Patricia

AU - García-Urkia, Nerea

AU - Irastorza, Igor

AU - Fernandez-San-Argimiro, Francisco Javier

AU - Uribe-Etxebarria, Verónica

AU - Jiménez-Rojo, Lucía

AU - Unda, Fernando

AU - Olalde, Beatriz

AU - Pineda, Jose Ramon

AU - Ibarretxe, Gaskon

PY - 2025

Y1 - 2025

N2 - Human Dental Pulp Stem Cells (hDPSCs) represent a remarkable cell source for tissue engineering and regenerative medicine, offering significant potential for use in personalized medicine and autologous therapies. Decellularized extracellular matrix (ECM)-derived biological scaffolds show excellent properties for supporting cell delivery and growth in both in vitro and in vivo applications. These scaffolds provide essential biochemical cues that regulate cellular functions and offer a more accurate representation of the in vivo environment. Porcine decellularized adipose tissue (pDAT) is a very abundant source of ECM, constituting an ideal material for biologic scaffold preparation. The integration of hDPSCs with pDAT-derived ECM enables the patient-specific generation of diverse humanized tissues and their application in personalized drug screening platforms. This chapter details a three dimensional (3D) culture methodology utilizing hDPSCs and pDAT-derived scaffolds to engineer humanized bone tissue.

AB - Human Dental Pulp Stem Cells (hDPSCs) represent a remarkable cell source for tissue engineering and regenerative medicine, offering significant potential for use in personalized medicine and autologous therapies. Decellularized extracellular matrix (ECM)-derived biological scaffolds show excellent properties for supporting cell delivery and growth in both in vitro and in vivo applications. These scaffolds provide essential biochemical cues that regulate cellular functions and offer a more accurate representation of the in vivo environment. Porcine decellularized adipose tissue (pDAT) is a very abundant source of ECM, constituting an ideal material for biologic scaffold preparation. The integration of hDPSCs with pDAT-derived ECM enables the patient-specific generation of diverse humanized tissues and their application in personalized drug screening platforms. This chapter details a three dimensional (3D) culture methodology utilizing hDPSCs and pDAT-derived scaffolds to engineer humanized bone tissue.

KW - 3D culture

KW - Adipose tissue

KW - Biologic scaffold

KW - Cell differentiation

KW - Decellularization

KW - Extracellular matrix

KW - Human dental pulp stem cells

KW - Osteogenesis

KW - Solid foam

KW - Tissue engineering

UR - https://www.scopus.com/pages/publications/105010914994

U2 - 10.1016/bs.mcb.2025.06.003

DO - 10.1016/bs.mcb.2025.06.003

M3 - Article

AN - SCOPUS:105010914994

SN - 0091-679X

JO - Methods in Cell Biology

JF - Methods in Cell Biology

ER -

Generation of humanized bone for disease modeling using porcine adipose tissue-derived extracellular matrix scaffolds and human dental pulp stem cells

Abstract

Keywords

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