Human α-thrombin detection platform using aptamers on a silicon nanowire field-effect transistor

  • Lotta Römhildt
  • , Felix Zörgiebel
  • , Bergoi Ibarlucea
  • , Maryam Vahdatzadeh
  • , Larysa Baraban
  • , Gianaurelio Cuniberti
  • , Sebastian Pregl
  • , Walter M. Weber
  • , Thomas Mikolajick
  • , Jörg Opitz

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

3 Citations (Scopus)

Abstract

We present a silicon nanowire-based field-effect transistor biosensor with Schottky barriers for highly specific and sensitive human α-thrombin detection. The active sensor area is decorated with thrombin-binding aptamers as receptor molecules. Each sensor chip is integrated into a microfluidic device for flow-through measurements. Instantaneous detection is provided by real-time monitoring of FET transfer curves. With this approach, thrombin concentrations between 200 pM and 200 nM are detected in a label-free, real-time manner, covering a wide dynamic range and enabling to distinguish between healthy and pathological levels. The development of simple, miniaturized devices for blood protein detection is highly interesting for medical diagnostics.

Original languageEnglish
Title of host publication2017 27th International Symposium on Power and Timing Modeling, Optimization and Simulation, PATMOS 2017
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1-4
Number of pages4
ISBN (Electronic)9781509064625
DOIs
Publication statusPublished - 13 Nov 2017
Externally publishedYes
Event27th International Symposium on Power and Timing Modeling, Optimization and Simulation, PATMOS 2017 - Thessaloniki, Greece
Duration: 25 Sept 201727 Sept 2017

Publication series

Name2017 27th International Symposium on Power and Timing Modeling, Optimization and Simulation, PATMOS 2017
Volume2017-January

Conference

Conference27th International Symposium on Power and Timing Modeling, Optimization and Simulation, PATMOS 2017
Country/TerritoryGreece
CityThessaloniki
Period25/09/1727/09/17

Keywords

  • Aptamers
  • Aptasensors
  • Field effect transistor
  • Human α-thrombin
  • Silicon nanowires

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