Label-free SERS for rapid identification of interleukin 6 based on intrinsic SERS fingerprint of antibody‑gold nanoparticles conjugate

Marjan Majdinasab; Aicha Azziz; Qiqian Liu; Verónica Mora-Sanz; Nerea Briz; Mathieu Edely; Marc Lamy de la Chapellea

doi:10.1016/j.ijbiomac.2023.127560

Label-free SERS for rapid identification of interleukin 6 based on intrinsic SERS fingerprint of antibody‑gold nanoparticles conjugate

Marjan Majdinasab, Aicha Azziz, Qiqian Liu, Verónica Mora-Sanz, Nerea Briz, Mathieu Edely, Marc Lamy de la Chapellea^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

A label-free surface-enhanced Raman scattering (SERS) was designed for sensitive detection of interleukin-6 (IL-6). The sensing element composed of anti-IL-6 antibodies adsorbed on the surface of spherical gold nanoparticles (AuNPs) as SERS-active surface. The principle of detection was probing antibody conformational changes using its intrinsic SERS fingerprint after binding to IL-6. Comparison of SERS spectra of antibody before and after binding to IL-6 showed that secondary structure of antibody does not change upon binding to IL-6. Vibrational information from disulfide bonds ν(S[sbnd]S) in antibody structure indicated some changes of geometry around S[sbnd]S bridges as a consequence of the immunocomplex formation. Transmission electron microscopy (TEM) and UV–Vis spectroscopy were used to confirm AuNPs conjugation with antibody as well as IL-6 binding to antibody on the surface of AuNPs. The SERS-based immunoassay showed a wide linear range (2.0–1000 pg mL⁻¹) and a high sensitivity with a limit of detection (LOD) as low as 0.91 pg mL⁻¹ (0.04 pM) without using any extrinsic Raman label. UV–Vis spectroscopy was employed as a conventional method for IL-6 detection based on observation of any change in the position of localized surface plasmon resonance (LSPR) band of AuNPs-antibody conjugates with LOD of 10 ng mL⁻¹.

Original language	English
Article number	127560
Journal	International Journal of Biological Macromolecules
Volume	253
DOIs	https://doi.org/10.1016/j.ijbiomac.2023.127560
Publication status	Published - 31 Dec 2023

Funding

This work was supported by the European project DeDNAed (H2020-FETOPEN2018-2020, no. 964248 ).

Keywords

Fingerprint
Interleukin 6
Surface-enhanced Raman scattering

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10.1016/j.ijbiomac.2023.127560

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title = "Label-free SERS for rapid identification of interleukin 6 based on intrinsic SERS fingerprint of antibody‑gold nanoparticles conjugate",

abstract = "A label-free surface-enhanced Raman scattering (SERS) was designed for sensitive detection of interleukin-6 (IL-6). The sensing element composed of anti-IL-6 antibodies adsorbed on the surface of spherical gold nanoparticles (AuNPs) as SERS-active surface. The principle of detection was probing antibody conformational changes using its intrinsic SERS fingerprint after binding to IL-6. Comparison of SERS spectra of antibody before and after binding to IL-6 showed that secondary structure of antibody does not change upon binding to IL-6. Vibrational information from disulfide bonds ν(S[sbnd]S) in antibody structure indicated some changes of geometry around S[sbnd]S bridges as a consequence of the immunocomplex formation. Transmission electron microscopy (TEM) and UV–Vis spectroscopy were used to confirm AuNPs conjugation with antibody as well as IL-6 binding to antibody on the surface of AuNPs. The SERS-based immunoassay showed a wide linear range (2.0–1000 pg mL−1) and a high sensitivity with a limit of detection (LOD) as low as 0.91 pg mL−1 (0.04 pM) without using any extrinsic Raman label. UV–Vis spectroscopy was employed as a conventional method for IL-6 detection based on observation of any change in the position of localized surface plasmon resonance (LSPR) band of AuNPs-antibody conjugates with LOD of 10 ng mL−1.",

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AU - Majdinasab, Marjan

AU - Azziz, Aicha

AU - Liu, Qiqian

AU - Mora-Sanz, Verónica

AU - Briz, Nerea

AU - Edely, Mathieu

AU - Lamy de la Chapellea, Marc

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AB - A label-free surface-enhanced Raman scattering (SERS) was designed for sensitive detection of interleukin-6 (IL-6). The sensing element composed of anti-IL-6 antibodies adsorbed on the surface of spherical gold nanoparticles (AuNPs) as SERS-active surface. The principle of detection was probing antibody conformational changes using its intrinsic SERS fingerprint after binding to IL-6. Comparison of SERS spectra of antibody before and after binding to IL-6 showed that secondary structure of antibody does not change upon binding to IL-6. Vibrational information from disulfide bonds ν(S[sbnd]S) in antibody structure indicated some changes of geometry around S[sbnd]S bridges as a consequence of the immunocomplex formation. Transmission electron microscopy (TEM) and UV–Vis spectroscopy were used to confirm AuNPs conjugation with antibody as well as IL-6 binding to antibody on the surface of AuNPs. The SERS-based immunoassay showed a wide linear range (2.0–1000 pg mL−1) and a high sensitivity with a limit of detection (LOD) as low as 0.91 pg mL−1 (0.04 pM) without using any extrinsic Raman label. UV–Vis spectroscopy was employed as a conventional method for IL-6 detection based on observation of any change in the position of localized surface plasmon resonance (LSPR) band of AuNPs-antibody conjugates with LOD of 10 ng mL−1.

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Label-free SERS for rapid identification of interleukin 6 based on intrinsic SERS fingerprint of antibody‑gold nanoparticles conjugate

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