TY - GEN
T1 - Low-cost electrospun nanofibers for label-free and real-time optical biodetection purposes
AU - Martínez-Pérez, Paula
AU - Ponce-Alcántara, Salvador
AU - Murillo, Nieves
AU - Pérez-Márquez, Ana
AU - Maudes, Jon
AU - Peraile, Inés
AU - González-López, Laura
AU - Gil-García, Matilde
AU - Lorenzo-Lozano, Paloma
AU - García-Rupérez, Jaime
N1 - Publisher Copyright:
© 2021 SPIE. All rights reserved.
PY - 2021
Y1 - 2021
N2 - In the development of label-free and real-time optical biosensors, a straightforward and low-cost fabrication of the transducer is important, because it reduces costs and the complexity of the process. One possibility for such easy fabrication is electrospinning. It is a versatile and well-developed technique that allows the manufacturing of layers of nanofibers at a low cost with a Fabry-Pérot cavity-based optical response when illuminated with visible light. In this research, the suitability of such layers for the development of label-free and real-time optical biosensors is studied. For such aim, the nanofibers were biofunctionalized in flow with antibodies against bovine serum albumin through an intermediate layer of protein A/G. Then, BSA was flowed at a concentration of 10 μg/ml as the target analyte. As a result, biofunctionalization and biodetection processes were optically monitored in real-time successfully, demonstrating the suitability of such a simple-to-fabricate transducer for the development of label-free and real-time optical biosensors. Furthermore, in comparison with other optical transducers that require complex nanofabrication techniques, electrospun nanofibers can be deposited over vast areas to create several transducers in a single batch and at a low cost.
AB - In the development of label-free and real-time optical biosensors, a straightforward and low-cost fabrication of the transducer is important, because it reduces costs and the complexity of the process. One possibility for such easy fabrication is electrospinning. It is a versatile and well-developed technique that allows the manufacturing of layers of nanofibers at a low cost with a Fabry-Pérot cavity-based optical response when illuminated with visible light. In this research, the suitability of such layers for the development of label-free and real-time optical biosensors is studied. For such aim, the nanofibers were biofunctionalized in flow with antibodies against bovine serum albumin through an intermediate layer of protein A/G. Then, BSA was flowed at a concentration of 10 μg/ml as the target analyte. As a result, biofunctionalization and biodetection processes were optically monitored in real-time successfully, demonstrating the suitability of such a simple-to-fabricate transducer for the development of label-free and real-time optical biosensors. Furthermore, in comparison with other optical transducers that require complex nanofabrication techniques, electrospun nanofibers can be deposited over vast areas to create several transducers in a single batch and at a low cost.
KW - Fabry-Pérot cavity
KW - Label-free biosensing
KW - Nanofibers
KW - Optical biosensor
KW - Porous materials
UR - http://www.scopus.com/inward/record.url?scp=85108460744&partnerID=8YFLogxK
U2 - 10.1117/12.2576527
DO - 10.1117/12.2576527
M3 - Conference contribution
AN - SCOPUS:85108460744
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVIII
A2 - Fixler, Dror
A2 - Goldys, Ewa M.
A2 - Wachsmann-Hogiu, Sebastian
PB - SPIE
T2 - Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVIII 2021
Y2 - 6 March 2021 through 11 March 2021
ER -
