Novel Concept of Micro Patterned Micro Titer Plates Fabricated via UV-NIL for Automated Neuronal Cell Assay Read-Out

Mirko Lohse; Manuel W. Thesen; Anja Haase; Martin Smolka; Nerea Briz Iceta; Ana Ayerdi Izquierdo; Isbaal Ramos; Clarisa Salado; Arne Schleunitz

doi:10.3390/nano11040902

Novel Concept of Micro Patterned Micro Titer Plates Fabricated via UV-NIL for Automated Neuronal Cell Assay Read-Out

Mirko Lohse, Manuel W. Thesen, Anja Haase, Martin Smolka, Nerea Briz Iceta, Ana Ayerdi Izquierdo, Isbaal Ramos, Clarisa Salado, Arne Schleunitz

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The UV-nanoimprint lithography(UV-NIL) fabrication of a novel network of micron-sized channels, forming an open channel microfluidic system is described. Details about the complete manufacturing process, from mastering to fabrication in small batches and in high throughput with up to 1200 micro titer plates per hour is presented. Deep insight into the evaluation of a suitable UV-curable material, mr-UVCur26SF is given, presenting cytotoxic evaluation, cell compatibility tests and finally a neuronal assay. The results indicate how the given pattern, in combination with the resist, paves the way to faster, cheaper, and more reliable drug screening.

Original language	English
Article number	902
Pages (from-to)	902
Number of pages	1
Journal	Nanomaterials
Volume	11
Issue number	4
DOIs	https://doi.org/10.3390/nano11040902
Publication status	Published - 1 Apr 2021

Keywords

Nanoimprint lithography
R2R UV-NIL
Neuronal cell assay

Project and Funding Information

Project ID
info:eu-repo/grantAgreement/EC/H2020/646260/EU/Large scale micro-and nanofabrication technologies for bioanalytical devices based on R2R imprinting/R2R Biofluidics
info:eu-repo/grantAgreement/EC/H2020/862092/EU/Next generation test bed for upscaling of microfluidic devices based on nano-enabled surfaces and membranes/NextGenMicrofluidics
Funding Info
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement NO. 646260. The research was also partially supported by NextGenMicrofluidics project under HORIZON2020 with grant agreement no 862092.

Access to Document

10.3390/nano11040902

Nanomaterials-11-00902Final published version, 1.14 MB

Cite this

@article{00a51fe6ff204471890e400adf282462,

title = "Novel Concept of Micro Patterned Micro Titer Plates Fabricated via UV-NIL for Automated Neuronal Cell Assay Read-Out",

abstract = "The UV-nanoimprint lithography(UV-NIL) fabrication of a novel network of micron-sized channels, forming an open channel microfluidic system is described. Details about the complete manufacturing process, from mastering to fabrication in small batches and in high throughput with up to 1200 micro titer plates per hour is presented. Deep insight into the evaluation of a suitable UV-curable material, mr-UVCur26SF is given, presenting cytotoxic evaluation, cell compatibility tests and finally a neuronal assay. The results indicate how the given pattern, in combination with the resist, paves the way to faster, cheaper, and more reliable drug screening.",

keywords = "Nanoimprint lithography, R2R UV-NIL, Neuronal cell assay, Nanoimprint lithography, R2R UV-NIL, Neuronal cell assay",

author = "Mirko Lohse and Thesen, {Manuel W.} and Anja Haase and Martin Smolka and Iceta, {Nerea Briz} and {Ayerdi Izquierdo}, Ana and Isbaal Ramos and Clarisa Salado and Arne Schleunitz",

note = "Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2021",

month = apr,

day = "1",

doi = "10.3390/nano11040902",

language = "English",

volume = "11",

pages = "902",

journal = "Nanomaterials",

issn = "2079-4991",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "4",

}

TY - JOUR

T1 - Novel Concept of Micro Patterned Micro Titer Plates Fabricated via UV-NIL for Automated Neuronal Cell Assay Read-Out

AU - Lohse, Mirko

AU - Thesen, Manuel W.

AU - Haase, Anja

AU - Smolka, Martin

AU - Iceta, Nerea Briz

AU - Ayerdi Izquierdo, Ana

AU - Ramos, Isbaal

AU - Salado, Clarisa

AU - Schleunitz, Arne

PY - 2021/4/1

Y1 - 2021/4/1

N2 - The UV-nanoimprint lithography(UV-NIL) fabrication of a novel network of micron-sized channels, forming an open channel microfluidic system is described. Details about the complete manufacturing process, from mastering to fabrication in small batches and in high throughput with up to 1200 micro titer plates per hour is presented. Deep insight into the evaluation of a suitable UV-curable material, mr-UVCur26SF is given, presenting cytotoxic evaluation, cell compatibility tests and finally a neuronal assay. The results indicate how the given pattern, in combination with the resist, paves the way to faster, cheaper, and more reliable drug screening.

AB - The UV-nanoimprint lithography(UV-NIL) fabrication of a novel network of micron-sized channels, forming an open channel microfluidic system is described. Details about the complete manufacturing process, from mastering to fabrication in small batches and in high throughput with up to 1200 micro titer plates per hour is presented. Deep insight into the evaluation of a suitable UV-curable material, mr-UVCur26SF is given, presenting cytotoxic evaluation, cell compatibility tests and finally a neuronal assay. The results indicate how the given pattern, in combination with the resist, paves the way to faster, cheaper, and more reliable drug screening.

KW - Nanoimprint lithography

KW - R2R UV-NIL

KW - Neuronal cell assay

KW - Nanoimprint lithography

KW - R2R UV-NIL

KW - Neuronal cell assay

UR - http://www.scopus.com/inward/record.url?scp=85103394776&partnerID=8YFLogxK

U2 - 10.3390/nano11040902

DO - 10.3390/nano11040902

M3 - Article

SN - 2079-4991

VL - 11

SP - 902

JO - Nanomaterials

JF - Nanomaterials

IS - 4

M1 - 902

ER -

Novel Concept of Micro Patterned Micro Titer Plates Fabricated via UV-NIL for Automated Neuronal Cell Assay Read-Out

Abstract

Keywords

Project and Funding Information

Access to Document

Other files and links

Fingerprint

Cite this