Numerically simulated pH-induced reactivation of catalytic activity of horseradish peroxidase

Ana Popović-Bijelić; Goran Bijelić; Ljiljana Kolar-Anić; Vladana Vukojević

doi:10.1196/annals.1342.066

Numerically simulated pH-induced reactivation of catalytic activity of horseradish peroxidase

Ana Popović-Bijelić
, Goran Bijelić
, Ljiljana Kolar-Anić
, Vladana Vukojević^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

A model mechanism that accounts for the experimentally observed pH-dependent enhancement of enzymatic activity of horseradish peroxidase through the catalase-like pathway is proposed. Predictions of the model are tested against a number of experimental results to confirm that kinetic constants used in the numerical simulation are correctly chosen and that the model can be used to emulate the reaction between horseradish peroxidase and hydrogen peroxide in a wide range of conditions.

Original language	English
Pages (from-to)	457-460
Number of pages	4
Journal	Annals of the New York Academy of Sciences
Volume	1048
DOIs	https://doi.org/10.1196/annals.1342.066
Publication status	Published - 2005
Externally published	Yes

Keywords

Catalase-like activity
Horseradish peroxidase
Numerical simulations
pH-induced reactivation of catalytic activity

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10.1196/annals.1342.066

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title = "Numerically simulated pH-induced reactivation of catalytic activity of horseradish peroxidase",

abstract = "A model mechanism that accounts for the experimentally observed pH-dependent enhancement of enzymatic activity of horseradish peroxidase through the catalase-like pathway is proposed. Predictions of the model are tested against a number of experimental results to confirm that kinetic constants used in the numerical simulation are correctly chosen and that the model can be used to emulate the reaction between horseradish peroxidase and hydrogen peroxide in a wide range of conditions.",

keywords = "Catalase-like activity, Horseradish peroxidase, Numerical simulations, pH-induced reactivation of catalytic activity",

author = "Ana Popovi{\'c}-Bijeli{\'c} and Goran Bijeli{\'c} and Ljiljana Kolar-Ani{\'c} and Vladana Vukojevi{\'c}",

year = "2005",

doi = "10.1196/annals.1342.066",

language = "English",

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pages = "457--460",

journal = "Annals of the New York Academy of Sciences",

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T1 - Numerically simulated pH-induced reactivation of catalytic activity of horseradish peroxidase

AU - Popović-Bijelić, Ana

AU - Bijelić, Goran

AU - Kolar-Anić, Ljiljana

AU - Vukojević, Vladana

PY - 2005

Y1 - 2005

N2 - A model mechanism that accounts for the experimentally observed pH-dependent enhancement of enzymatic activity of horseradish peroxidase through the catalase-like pathway is proposed. Predictions of the model are tested against a number of experimental results to confirm that kinetic constants used in the numerical simulation are correctly chosen and that the model can be used to emulate the reaction between horseradish peroxidase and hydrogen peroxide in a wide range of conditions.

AB - A model mechanism that accounts for the experimentally observed pH-dependent enhancement of enzymatic activity of horseradish peroxidase through the catalase-like pathway is proposed. Predictions of the model are tested against a number of experimental results to confirm that kinetic constants used in the numerical simulation are correctly chosen and that the model can be used to emulate the reaction between horseradish peroxidase and hydrogen peroxide in a wide range of conditions.

KW - Catalase-like activity

KW - Horseradish peroxidase

KW - Numerical simulations

KW - pH-induced reactivation of catalytic activity

UR - https://www.scopus.com/pages/publications/25144516627

U2 - 10.1196/annals.1342.066

DO - 10.1196/annals.1342.066

M3 - Article

C2 - 16154977

AN - SCOPUS:25144516627

SN - 0077-8923

VL - 1048

SP - 457

EP - 460

JO - Annals of the New York Academy of Sciences

JF - Annals of the New York Academy of Sciences

ER -

Numerically simulated pH-induced reactivation of catalytic activity of horseradish peroxidase

Abstract

Keywords

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