TY - GEN
T1 - Photonic bioreactors based on multiple internal reflection
AU - Ibarlucea, Bergoi
AU - Cadarso, Victor Javier
AU - Orozco, Jahir
AU - Demming, Stefanie
AU - Fernández-Sánchez, Cesar
AU - Wilke, Ralph
AU - Büttgenbach, Stephanus
AU - Llobera, Andreu
PY - 2009
Y1 - 2009
N2 - Propagating Multiple Internal Reflection (PMIR) systems have been functionalized with horseradish peroxidase (HRP) so as to obtain highly sensitive PDMS-based photonic bioreactors in which photonic, microfluidcs and recognition elements are merged in a single lab on a chip. Demonstration of the working principle was done by making absorbance measurements in ABTS solutions with increasing hydrogen peroxide concentrations, which allowed determining a sensitivity of 0.025±0.004 (a.u.)/μM and a LOD of 2.8±0.4 μM.
AB - Propagating Multiple Internal Reflection (PMIR) systems have been functionalized with horseradish peroxidase (HRP) so as to obtain highly sensitive PDMS-based photonic bioreactors in which photonic, microfluidcs and recognition elements are merged in a single lab on a chip. Demonstration of the working principle was done by making absorbance measurements in ABTS solutions with increasing hydrogen peroxide concentrations, which allowed determining a sensitivity of 0.025±0.004 (a.u.)/μM and a LOD of 2.8±0.4 μM.
KW - Biofunctionalization
KW - PDMS
KW - PMIR
KW - Photonic lab-on-a-chip
UR - https://www.scopus.com/pages/publications/84901773608
M3 - Conference contribution
AN - SCOPUS:84901773608
SN - 9780979806421
T3 - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 287
EP - 289
BT - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
Y2 - 1 November 2009 through 5 November 2009
ER -