TY - JOUR
T1 - Positive selection results in frequent reversible amino acid replacements in the G protein gene of human respiratory syncytial virus
AU - Botosso, Viviane F.
AU - Zanotto, Paolo M.De A.
AU - Ueda, Mirthes
AU - Arruda, Eurico
AU - Gilio, Alfredo E.
AU - Ieira, Sandra E.
AU - Stewien, Klaus E.
AU - Peret, Teresa C.T.
AU - Jamal, Leda F.
AU - Pardini, Maria I.De M.C.
AU - Pinho, Joãr
AU - Massad, Duardo
AU - Sant'Anna, Osvaldo A.
AU - Holmes, Eddie C.
AU - Durigon, Edison L.
AU - Comone, Priscila
AU - Do Sacramento, Patrícia R.
AU - Durigan, Mariana S.
AU - Oliveira, Danielle B.L.
AU - Moraes, Claudia T.P.
AU - Campos, Angélica Cristine De Almeida
AU - Leal, Andréia L.
AU - Silva, Tereza S.
AU - Carvalho, Ariane C.L.
AU - Tenório, Elisabeth C.N.
AU - Cintra, Otavio A.L.
AU - Ansarah-Sobrinho, Camilo
AU - Proençna-Modena, José L.
AU - Iwamoto, Marisa A.
AU - De Paula, Flávia E.
AU - Souza, Maria C.O.
AU - Vaz-de-Lima, Lourdes R.A.
AU - Matsumoto, Tokiko K.
AU - Sato, Neuza N.
AU - Salgado, Maristela M.
AU - Hong, Marisa A.
AU - Requejo, Henry I.
AU - Barbosa, Maria L.
AU - Oliveiveira, Carmem A.F.
AU - Passos, Saulo D.
AU - Pecchini, Rogério
AU - Berezin, Eitan
AU - Schvartsman, Claudio
AU - Pannuti, Cláudio S.
AU - Candeias, João M.G.
AU - Han, Sang W.
AU - Garcia, José F.
AU - Carrilho, Flair J.
AU - Figueiredo, Luíz T.M.
AU - Duarte, Alberto J.Da S.
AU - Wolff, José L.C.
AU - Rahal, Paula
AU - Richtzenhain, Leonardo J.
AU - Gonçales, Fernando L.
AU - De Lima, Edimo G.
PY - 2009/1/1
Y1 - 2009/1/1
N2 - Human respiratory syncytial virus (HRSV) is the major cause of lower respiratory tract infections in children under 5 years of age and the elderly, causing annual disease outbreaks during the fall and winter. Multiple lineages of the HRSVA and HRSVB serotypes co-circulate within a single outbreak and display a strongly temporal pattern of genetic variation, with a replacement of dominant genotypes occurring during consecutive years. In the present study we utilized phylogenetic methods to detect and map sites subject to adaptive evolution in the G protein of HRSVA and HRSVB. A total of 29 and 23 amino acid sites were found to be putatively positively selected in HRSVA and HRSVB, respectively. Several of these sites defined genotypes and lineages within genotypes in both groups, and correlated well with epitopes previously described in group A. Remarkably, 18 of these positively selected tended to revert in time to a previous codon state, producing a "flipflop" phylogenetic pattern. Such frequent evolutionary reversals in HRSV are indicative of a combination of frequent positive selection, reflecting the changing immune status of the human population, and a limited repertoire of functionally viable amino acids at specific amino acid sites.
AB - Human respiratory syncytial virus (HRSV) is the major cause of lower respiratory tract infections in children under 5 years of age and the elderly, causing annual disease outbreaks during the fall and winter. Multiple lineages of the HRSVA and HRSVB serotypes co-circulate within a single outbreak and display a strongly temporal pattern of genetic variation, with a replacement of dominant genotypes occurring during consecutive years. In the present study we utilized phylogenetic methods to detect and map sites subject to adaptive evolution in the G protein of HRSVA and HRSVB. A total of 29 and 23 amino acid sites were found to be putatively positively selected in HRSVA and HRSVB, respectively. Several of these sites defined genotypes and lineages within genotypes in both groups, and correlated well with epitopes previously described in group A. Remarkably, 18 of these positively selected tended to revert in time to a previous codon state, producing a "flipflop" phylogenetic pattern. Such frequent evolutionary reversals in HRSV are indicative of a combination of frequent positive selection, reflecting the changing immune status of the human population, and a limited repertoire of functionally viable amino acids at specific amino acid sites.
UR - http://www.scopus.com/inward/record.url?scp=59249085256&partnerID=8YFLogxK
U2 - 10.1371/journal.ppat.1000254
DO - 10.1371/journal.ppat.1000254
M3 - Article
C2 - 19119418
AN - SCOPUS:59249085256
SN - 1553-7366
VL - 5
JO - PLoS Pathogens
JF - PLoS Pathogens
IS - 1
M1 - e1000254
ER -
