TY - JOUR
T1 - Taenia saginata
T2 - Polymerase chain reaction for taeniasis diagnosis in human fecal samples
AU - Nunes, Cáris Maroni
AU - Lima, Luiz Gustave Ferraz
AU - Manoel, Camila Santos
AU - Pereira, Rodrigo Norberte
AU - Nakano, Mauro Massaham
AU - Garcia, José Fernando
PY - 2003
Y1 - 2003
N2 - The taeniasis-cysticercosis complex is a zoonosis of great medical and economic importance where humans play an important role as the carrier of adult stage of Taenia solium and Taenia saginata. This paper describes PCR standardization that can be applied in human fecal samples for taeniasis diagnosis. DNA extraction was achieved with DNAzol reagent, after egg disruption with glass beads. DNA prepared from fecal specimens was first purified and PCR amplified generating fragments of 170 and 600 bp. The assay described herein provides an important tool for T. saginata identification in human fecal samples.
AB - The taeniasis-cysticercosis complex is a zoonosis of great medical and economic importance where humans play an important role as the carrier of adult stage of Taenia solium and Taenia saginata. This paper describes PCR standardization that can be applied in human fecal samples for taeniasis diagnosis. DNA extraction was achieved with DNAzol reagent, after egg disruption with glass beads. DNA prepared from fecal specimens was first purified and PCR amplified generating fragments of 170 and 600 bp. The assay described herein provides an important tool for T. saginata identification in human fecal samples.
UR - https://www.scopus.com/pages/publications/0141669146
U2 - 10.1016/S0014-4894(03)00111-5
DO - 10.1016/S0014-4894(03)00111-5
M3 - Article
C2 - 12932762
AN - SCOPUS:0141669146
SN - 0014-4894
VL - 104
SP - 67
EP - 69
JO - Experimental Parasitology
JF - Experimental Parasitology
IS - 1-2
ER -