TY - JOUR
T1 - The DREAM-DRE interaction
T2 - Key nucleotides and dominant negative mutants
AU - Ledo, Fran
AU - Link, Wolfgang A.
AU - Carrión, Angel M.
AU - Echeverria, Valentina
AU - Mellström, Britt
AU - Naranjo, José R.
PY - 2000/12/20
Y1 - 2000/12/20
N2 - Transcriptional repressor DREAM, an EF-hand containing calcium-binding protein, blocks basal expression of target genes through specific interaction with DRE sites in the DNA. The sequence GTCA forms the central core of the DRE site, whereas flanking nucleotides contribute notably to the affinity for DREAM. Release of binding of DREAM from the DRE results in derepression, a process that is regulated by Ca2+. Change of two amino acids within an EF-hand in DREAM blocks Ca2+-induced derepression and results in potent dominant negative mutants of endogenous DREAM.
AB - Transcriptional repressor DREAM, an EF-hand containing calcium-binding protein, blocks basal expression of target genes through specific interaction with DRE sites in the DNA. The sequence GTCA forms the central core of the DRE site, whereas flanking nucleotides contribute notably to the affinity for DREAM. Release of binding of DREAM from the DRE results in derepression, a process that is regulated by Ca2+. Change of two amino acids within an EF-hand in DREAM blocks Ca2+-induced derepression and results in potent dominant negative mutants of endogenous DREAM.
KW - Ca-regulated transcription
KW - DRE
KW - DREAM
KW - Dominant negative mutant
KW - EF-hand
UR - https://www.scopus.com/pages/publications/0034695102
U2 - 10.1016/S0167-4889(00)00092-6
DO - 10.1016/S0167-4889(00)00092-6
M3 - Review article
C2 - 11108959
AN - SCOPUS:0034695102
SN - 0167-4889
VL - 1498
SP - 162
EP - 168
JO - Biochimica et Biophysica Acta - Molecular Cell Research
JF - Biochimica et Biophysica Acta - Molecular Cell Research
IS - 2-3
ER -