The DREAM-DRE interaction: Key nucleotides and dominant negative mutants

Fran Ledo; Wolfgang A. Link; Angel M. Carrión; Valentina Echeverria; Britt Mellström; José R. Naranjo

doi:10.1016/S0167-4889(00)00092-6

The DREAM-DRE interaction: Key nucleotides and dominant negative mutants

Fran Ledo
, Wolfgang A. Link
, Angel M. Carrión
, Valentina Echeverria
, Britt Mellström
, José R. Naranjo

Consejo Superior de Investigaciones Científicas (CSIC)

Research output: Contribution to journal › Review article › peer-review

44 Citations (Scopus)

Abstract

Transcriptional repressor DREAM, an EF-hand containing calcium-binding protein, blocks basal expression of target genes through specific interaction with DRE sites in the DNA. The sequence GTCA forms the central core of the DRE site, whereas flanking nucleotides contribute notably to the affinity for DREAM. Release of binding of DREAM from the DRE results in derepression, a process that is regulated by Ca²⁺. Change of two amino acids within an EF-hand in DREAM blocks Ca²⁺-induced derepression and results in potent dominant negative mutants of endogenous DREAM.

Original language	English
Pages (from-to)	162-168
Number of pages	7
Journal	Biochimica et Biophysica Acta - Molecular Cell Research
Volume	1498
Issue number	2-3
DOIs	https://doi.org/10.1016/S0167-4889(00)00092-6
Publication status	Published - 20 Dec 2000
Externally published	Yes

Keywords

Ca-regulated transcription
DRE
DREAM
Dominant negative mutant
EF-hand

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10.1016/S0167-4889(00)00092-6

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title = "The DREAM-DRE interaction: Key nucleotides and dominant negative mutants",

abstract = "Transcriptional repressor DREAM, an EF-hand containing calcium-binding protein, blocks basal expression of target genes through specific interaction with DRE sites in the DNA. The sequence GTCA forms the central core of the DRE site, whereas flanking nucleotides contribute notably to the affinity for DREAM. Release of binding of DREAM from the DRE results in derepression, a process that is regulated by Ca2+. Change of two amino acids within an EF-hand in DREAM blocks Ca2+-induced derepression and results in potent dominant negative mutants of endogenous DREAM.",

keywords = "Ca-regulated transcription, DRE, DREAM, Dominant negative mutant, EF-hand",

author = "Fran Ledo and Link, \{Wolfgang A.\} and Carri{\'o}n, \{Angel M.\} and Valentina Echeverria and Britt Mellstr{\"o}m and Naranjo, \{Jos{\'e} R.\}",

year = "2000",

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T1 - The DREAM-DRE interaction

T2 - Key nucleotides and dominant negative mutants

AU - Ledo, Fran

AU - Link, Wolfgang A.

AU - Carrión, Angel M.

AU - Echeverria, Valentina

AU - Mellström, Britt

AU - Naranjo, José R.

PY - 2000/12/20

Y1 - 2000/12/20

N2 - Transcriptional repressor DREAM, an EF-hand containing calcium-binding protein, blocks basal expression of target genes through specific interaction with DRE sites in the DNA. The sequence GTCA forms the central core of the DRE site, whereas flanking nucleotides contribute notably to the affinity for DREAM. Release of binding of DREAM from the DRE results in derepression, a process that is regulated by Ca2+. Change of two amino acids within an EF-hand in DREAM blocks Ca2+-induced derepression and results in potent dominant negative mutants of endogenous DREAM.

AB - Transcriptional repressor DREAM, an EF-hand containing calcium-binding protein, blocks basal expression of target genes through specific interaction with DRE sites in the DNA. The sequence GTCA forms the central core of the DRE site, whereas flanking nucleotides contribute notably to the affinity for DREAM. Release of binding of DREAM from the DRE results in derepression, a process that is regulated by Ca2+. Change of two amino acids within an EF-hand in DREAM blocks Ca2+-induced derepression and results in potent dominant negative mutants of endogenous DREAM.

KW - Ca-regulated transcription

KW - DRE

KW - DREAM

KW - Dominant negative mutant

KW - EF-hand

UR - https://www.scopus.com/pages/publications/0034695102

U2 - 10.1016/S0167-4889(00)00092-6

DO - 10.1016/S0167-4889(00)00092-6

M3 - Review article

C2 - 11108959

AN - SCOPUS:0034695102

SN - 0167-4889

VL - 1498

SP - 162

EP - 168

JO - Biochimica et Biophysica Acta - Molecular Cell Research

JF - Biochimica et Biophysica Acta - Molecular Cell Research

IS - 2-3

ER -

The DREAM-DRE interaction: Key nucleotides and dominant negative mutants

Abstract

Keywords

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