Thrombin detection by means of an aptamer based sensitive coating fabricated onto LMR-based optical fiber refractometer

  • L. Razquin*
  • , C. R. Zamarreno
  • , F. J. Munoz
  • , I. R. Matias
  • , F. J. Arregui
  • *Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

25 Citations (Scopus)

Abstract

An optical fiber sensor using an aptamer receptor has been developed for the detection of thrombin. The sensitive coating consists of an aptamer-polymer structure fabricated using the layer-by-layer electrostatic self assembly technique onto novel optical fiber refractometers based on lossy mode resonances (LMRs). LMR based refractometers enable to overcome the traditional drawbacks of surface Plasmon resonance (SPR) based devices, such as the utilization of polarized light for example. The refractometer is based on an indium tin oxide thin layer deposited by DC-sputtering onto the cladding removed multimode fiber. These refractometers enable to monitor the variations of the surrounding medium refractive index (RI). Hence, RI variations of the outer film induced by thrombin can be referenced to the LMR wavelength shift. The described configuration has enabled the detection of low concentrations of thrombin (100nM) as well as obtaining a maximum wavelength shift of 50 nm at higher (1μM) concentrations.

Original languageEnglish
Title of host publicationIEEE SENSORS 2012 - Proceedings
DOIs
Publication statusPublished - 2012
Externally publishedYes
Event11th IEEE SENSORS 2012 Conference - Taipei, Taiwan, Province of China
Duration: 28 Oct 201231 Oct 2012

Publication series

NameProceedings of IEEE Sensors

Conference

Conference11th IEEE SENSORS 2012 Conference
Country/TerritoryTaiwan, Province of China
CityTaipei
Period28/10/1231/10/12

Fingerprint

Dive into the research topics of 'Thrombin detection by means of an aptamer based sensitive coating fabricated onto LMR-based optical fiber refractometer'. Together they form a unique fingerprint.

Cite this