Upgrading the organic fraction of municipal solid waste to poly(3-hydroxybutyrate)

Jon Kepa Izaguirre; M. Manuela R. da Fonseca; Pedro Fernandes; M. Carmen Villarán; Sonia Castañón; M. Teresa Cesário

doi:10.1016/j.biortech.2019.121785

Upgrading the organic fraction of municipal solid waste to poly(3-hydroxybutyrate)

Jon Kepa Izaguirre^*, M. Manuela R. da Fonseca, Pedro Fernandes, M. Carmen Villarán, Sonia Castañón, M. Teresa Cesário

^*Corresponding author for this work

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Abstract

The organic fraction of municipal solid waste was studied as feedstock for the production of poly(3-hydroxybutyrate) (P(3HB)). To release the monosaccharides, a diluted acid pre-treatment followed by an enzymatic hydrolysis was applied. A sugar yield of 49% was achieved using a pre-treated waste and an enzyme cocktail of Pentopan 500 BG and Celluclast BG. The addition of Glucoamylase NS 22035 helped to hydrolyze the starch fraction, improving the hydrolysis yield to 56%. The hydrolysate was used as culture medium to produce P(3HB) by Burkholderia sacchari DSM 17165. Assays at shaking flask scale showed that when the hydrolysate was used as substrate, the attained cell concentration was slightly higher than in the control medium. It was necessary to supplement the hydrolysate with extra glucose to increase the C/N ratio and with a mineral solution to overcome the nutritional deficiencies. The P(3HB) accumulation using the supplemented hydrolysate was 58% (g polymer/g biomass).

Original language	English
Article number	121785
Journal	Bioresource Technology
Volume	290
DOIs	https://doi.org/10.1016/j.biortech.2019.121785
Publication status	Published - Oct 2019

Funding

The authors are thankful for the technical and human support provided by SGIker of UPV/EHU and for the European funding ( ERDF and ESF ). Funding received by iBB-Institute for Bioengineering and Biosciences from FCT -Portuguese Foundation for Science and Technology (grant UID/BIO/04565/2013 ) and from Programa Operacional Regional de Lisboa 2020 (Project N. 007317) is acknowledged. Jon Kepa Izaguirre would like to acknowledge the Basque Government for awarding a predoctoral fellowship to achieve his PhD degree in the context of this research project. The authors are thankful for the technical and human support provided by SGIker of UPV/EHU and for the European funding (ERDF and ESF). Funding received by iBB-Institute for Bioengineering and Biosciences from FCT-Portuguese Foundation for Science and Technology (grant UID/BIO/04565/2013) and from Programa Operacional Regional de Lisboa 2020 (Project N. 007317) is acknowledged. Jon Kepa Izaguirre would like to acknowledge the Basque Government for awarding a predoctoral fellowship to achieve his PhD degree in the context of this research project.

Funders	Funder number
Programa Operacional Regional de Lisboa 2020	007317
Fuel Cell Technologies Office
Fundação para a Ciência e a Tecnologia	UID/BIO/04565/2013
Eusko Jaurlaritza
European Regional Development Fund	ESF
Fundació Catalana de Trasplantament
Institute for Bioengineering and Biosciences

Keywords

Burkholderia sacchari
Diluted acid pre-treatment
Enzymatic hydrolysis
Municipal solid waste
Poly(3-hydroxybutyrate)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2019.121785

Cite this

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abstract = "The organic fraction of municipal solid waste was studied as feedstock for the production of poly(3-hydroxybutyrate) (P(3HB)). To release the monosaccharides, a diluted acid pre-treatment followed by an enzymatic hydrolysis was applied. A sugar yield of 49% was achieved using a pre-treated waste and an enzyme cocktail of Pentopan 500 BG and Celluclast BG. The addition of Glucoamylase NS 22035 helped to hydrolyze the starch fraction, improving the hydrolysis yield to 56%. The hydrolysate was used as culture medium to produce P(3HB) by Burkholderia sacchari DSM 17165. Assays at shaking flask scale showed that when the hydrolysate was used as substrate, the attained cell concentration was slightly higher than in the control medium. It was necessary to supplement the hydrolysate with extra glucose to increase the C/N ratio and with a mineral solution to overcome the nutritional deficiencies. The P(3HB) accumulation using the supplemented hydrolysate was 58% (g polymer/g biomass).",

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AU - Villarán, M. Carmen

AU - Castañón, Sonia

AU - Cesário, M. Teresa

PY - 2019/10

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N2 - The organic fraction of municipal solid waste was studied as feedstock for the production of poly(3-hydroxybutyrate) (P(3HB)). To release the monosaccharides, a diluted acid pre-treatment followed by an enzymatic hydrolysis was applied. A sugar yield of 49% was achieved using a pre-treated waste and an enzyme cocktail of Pentopan 500 BG and Celluclast BG. The addition of Glucoamylase NS 22035 helped to hydrolyze the starch fraction, improving the hydrolysis yield to 56%. The hydrolysate was used as culture medium to produce P(3HB) by Burkholderia sacchari DSM 17165. Assays at shaking flask scale showed that when the hydrolysate was used as substrate, the attained cell concentration was slightly higher than in the control medium. It was necessary to supplement the hydrolysate with extra glucose to increase the C/N ratio and with a mineral solution to overcome the nutritional deficiencies. The P(3HB) accumulation using the supplemented hydrolysate was 58% (g polymer/g biomass).

AB - The organic fraction of municipal solid waste was studied as feedstock for the production of poly(3-hydroxybutyrate) (P(3HB)). To release the monosaccharides, a diluted acid pre-treatment followed by an enzymatic hydrolysis was applied. A sugar yield of 49% was achieved using a pre-treated waste and an enzyme cocktail of Pentopan 500 BG and Celluclast BG. The addition of Glucoamylase NS 22035 helped to hydrolyze the starch fraction, improving the hydrolysis yield to 56%. The hydrolysate was used as culture medium to produce P(3HB) by Burkholderia sacchari DSM 17165. Assays at shaking flask scale showed that when the hydrolysate was used as substrate, the attained cell concentration was slightly higher than in the control medium. It was necessary to supplement the hydrolysate with extra glucose to increase the C/N ratio and with a mineral solution to overcome the nutritional deficiencies. The P(3HB) accumulation using the supplemented hydrolysate was 58% (g polymer/g biomass).

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Upgrading the organic fraction of municipal solid waste to poly(3-hydroxybutyrate)

Abstract

Funding

Keywords

UN SDGs

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