3D additive manufactured composite scaffolds with antibiotic-loaded lamellar fillers for bone infection prevention and tissue regeneration

María Cámara-Torres, Stacy Duarte, Ravi Sinha, Ainhoa Egizabal, Noelia Álvarez, Maria Bastianini, Michele Sisani, Paolo Scopece, Marco Scatto, Alessandro Bonetto, Antonio Marcomini, Alberto Sanchez, Alessandro Patelli, Carlos Mota, Lorenzo Moroni

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

58 Citas (Scopus)
2 Descargas (Pure)

Resumen

Bone infections following open bone fracture or implant surgery remain a challenge in the orthopedics field. In order to avoid high doses of systemic drug administration, optimized local antibiotic release from scaffolds is required. 3D additive manufactured (AM) scaffolds made with biodegradable polymers are ideal to support bone healing in non-union scenarios and can be given antimicrobial properties by the incorporation of antibiotics. In this study, ciprofloxacin and gentamicin intercalated in the interlamellar spaces of magnesium aluminum layered double hydroxides (MgAl) and α-zirconium phosphates (ZrP), respectively, are dispersed within a thermoplastic polymer by melt compounding and subsequently processed via high temperature melt extrusion AM (~190 °C) into 3D scaffolds. The inorganic fillers enable a sustained antibiotics release through the polymer matrix, controlled by antibiotics counterions exchange or pH conditions. Importantly, both antibiotics retain their functionality after the manufacturing process at high temperatures, as verified by their activity against both Gram + and Gram - bacterial strains. Moreover, scaffolds loaded with filler-antibiotic do not impair human mesenchymal stromal cells osteogenic differentiation, allowing matrix mineralization and the expression of relevant osteogenic markers. Overall, these results suggest the possibility of fabricating dual functionality 3D scaffolds via high temperature melt extrusion for bone regeneration and infection prevention.
Idioma originalInglés
Páginas (desde-hasta)1073-1082
Número de páginas10
PublicaciónBioactive Materials
Volumen6
N.º4
DOI
EstadoPublicada - abr 2021

Palabras clave

  • Melt extrusion additive manufacturing
  • Antibiotic delivery
  • Lamellar inorganic fillers
  • Bone infection
  • Bone regeneration
  • Human mesenchymal stromal cells

Project and Funding Information

  • Project ID
  • info:eu-repo/grantAgreement/EC/H2020/685825/EU/Functionally graded Additive Manufacturing scaffolds by hybrid manufacturing/FAST
  • Funding Info
  • We are grateful to the FAST project funded under the H2020-NMP- PILOTS-2015 scheme (GA n. 685825) for financial support. Some of the materials used in this work were provided by the Texas A&M Health Science Center College of Medicine Institute for Regenerative Medicine at Scott & White through a grant from NCRR of the NIH (Grant #P40RR017447).

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