TY - GEN
T1 - Biofunctionalized Lab-on-a-Chip with dual readout
AU - Ibarlucea, B.
AU - Munoz-Berbel, X.
AU - Ortiz, P.
AU - Büttgenbach, S.
AU - Fernández-Sánchez, C.
AU - Llobera, A.
PY - 2013
Y1 - 2013
N2 - A dual (electrochemical and optical) Lab-on-a-Chip is presented, which monolithically combines a Multiple Internal Reflection and a biofunctionalized mixer that also plays the role of reactor. Here, there is no need to include an enzyme reservoir, resulting in a reduction of dead volumes. This approach was validated by functionalizing the mixer/reactor with horseradish peroxidase and glucose oxidase, being possible to detect glucose optically and electrochemically under continuous flow conditions, with a limit of detection of 6.3 ± 0.4 mM at 100 μL/min (20 s response time) and 0.113 ±3 7times; 10-3 mM at 10 μL/min (1 min response time).
AB - A dual (electrochemical and optical) Lab-on-a-Chip is presented, which monolithically combines a Multiple Internal Reflection and a biofunctionalized mixer that also plays the role of reactor. Here, there is no need to include an enzyme reservoir, resulting in a reduction of dead volumes. This approach was validated by functionalizing the mixer/reactor with horseradish peroxidase and glucose oxidase, being possible to detect glucose optically and electrochemically under continuous flow conditions, with a limit of detection of 6.3 ± 0.4 mM at 100 μL/min (20 s response time) and 0.113 ±3 7times; 10-3 mM at 10 μL/min (1 min response time).
KW - Biofunctionalization
KW - Lab-on-a-Chip
KW - Microfluidics
KW - Photonic Lab-on-a-Chip
KW - Reactor
UR - https://www.scopus.com/pages/publications/84907330052
M3 - Conference contribution
AN - SCOPUS:84907330052
SN - 9781632666246
T3 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
SP - 1496
EP - 1498
BT - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PB - Chemical and Biological Microsystems Society
T2 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Y2 - 27 October 2013 through 31 October 2013
ER -