In vitro evaluation of combined laser processing on Ti6Al4V discs: Macrostructuring and pulsed laser deposition

Human fetal osteoblast (hFOB) cell adhesion and cell proliferation studies have been performed on laser macrostructured titanium alloy substrates with and without pulsed laser deposited thin calcium phosphate coating. Coating was only 100 nm thick. The combined laser processing approach for modification of titanium alloy surfaces has yielded improved cell adhesion and proliferation of cells. Alkaline phosphatase activity was very significantly higher in the coated samples suggesting a intense stimuli of early osteoblast differentiation that should lead to improve bone growth on these surfaces.