Novel Concept of Micro Patterned Micro Titer Plates Fabricated via UV-NIL for Automated Neuronal Cell Assay Read-Out

Mirko Lohse; Manuel W. Thesen; Anja Haase; Martin Smolka; Nerea Briz Iceta; Ana Ayerdi Izquierdo; Isbaal Ramos; Clarisa Salado; Arne Schleunitz

doi:10.3390/nano11040902

Novel Concept of Micro Patterned Micro Titer Plates Fabricated via UV-NIL for Automated Neuronal Cell Assay Read-Out

Mirko Lohse
, Manuel W. Thesen
, Anja Haase
, Martin Smolka
, Nerea Briz Iceta
, Ana Ayerdi Izquierdo
, Isbaal Ramos
, Clarisa Salado
, Arne Schleunitz

Micro Resist Technology GmbH
Joanneum Research
Innoprot SL

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

11 Citas (Scopus)

4 Descargas (Pure)

Resumen

The UV-nanoimprint lithography(UV-NIL) fabrication of a novel network of micron-sized channels, forming an open channel microfluidic system is described. Details about the complete manufacturing process, from mastering to fabrication in small batches and in high throughput with up to 1200 micro titer plates per hour is presented. Deep insight into the evaluation of a suitable UV-curable material, mr-UVCur26SF is given, presenting cytotoxic evaluation, cell compatibility tests and finally a neuronal assay. The results indicate how the given pattern, in combination with the resist, paves the way to faster, cheaper, and more reliable drug screening.

Idioma original	Inglés
Número de artículo	902
Páginas (desde-hasta)	902
Número de páginas	1
Publicación	Nanomaterials
Volumen	11
N.º	4
DOI	https://doi.org/10.3390/nano11040902
Estado	Publicada - 1 abr 2021

ODS de las Naciones Unidas

Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

ODS 9: Industria, innovación e infraestructura

Palabras clave

Nanoimprint lithography
R2R UV-NIL
Neuronal cell assay

Project and Funding Information

Project ID
info:eu-repo/grantAgreement/EC/H2020/646260/EU/Large scale micro-and nanofabrication technologies for bioanalytical devices based on R2R imprinting/R2R Biofluidics
info:eu-repo/grantAgreement/EC/H2020/862092/EU/Next generation test bed for upscaling of microfluidic devices based on nano-enabled surfaces and membranes/NextGenMicrofluidics
Funding Info
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement NO. 646260. The research was also partially supported by NextGenMicrofluidics project under HORIZON2020 with grant agreement no 862092.

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Nanomaterials-11-00902Versión publicada final, 1,14 MB

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title = "Novel Concept of Micro Patterned Micro Titer Plates Fabricated via UV-NIL for Automated Neuronal Cell Assay Read-Out",

abstract = "The UV-nanoimprint lithography(UV-NIL) fabrication of a novel network of micron-sized channels, forming an open channel microfluidic system is described. Details about the complete manufacturing process, from mastering to fabrication in small batches and in high throughput with up to 1200 micro titer plates per hour is presented. Deep insight into the evaluation of a suitable UV-curable material, mr-UVCur26SF is given, presenting cytotoxic evaluation, cell compatibility tests and finally a neuronal assay. The results indicate how the given pattern, in combination with the resist, paves the way to faster, cheaper, and more reliable drug screening.",

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AU - Lohse, Mirko

AU - Thesen, Manuel W.

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AU - Smolka, Martin

AU - Iceta, Nerea Briz

AU - Ayerdi Izquierdo, Ana

AU - Ramos, Isbaal

AU - Salado, Clarisa

AU - Schleunitz, Arne

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AB - The UV-nanoimprint lithography(UV-NIL) fabrication of a novel network of micron-sized channels, forming an open channel microfluidic system is described. Details about the complete manufacturing process, from mastering to fabrication in small batches and in high throughput with up to 1200 micro titer plates per hour is presented. Deep insight into the evaluation of a suitable UV-curable material, mr-UVCur26SF is given, presenting cytotoxic evaluation, cell compatibility tests and finally a neuronal assay. The results indicate how the given pattern, in combination with the resist, paves the way to faster, cheaper, and more reliable drug screening.

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Novel Concept of Micro Patterned Micro Titer Plates Fabricated via UV-NIL for Automated Neuronal Cell Assay Read-Out

Resumen

ODS de las Naciones Unidas

Palabras clave

Project and Funding Information

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