Selective cathodic microbial biofilm retention allows a high current-to-sulfide efficiency in sulfate-reducing microbial electrolysis cells

Selective microbial retention is of paramount importance for the long-term performance of cathodic sulfate reduction in microbial electrolysis cells (MECs) due to the slow growth rate of autotrophic sulfate-reducing bacteria. In this work, we investigate the biofilm retention and current-to-sulfide conversion efficiency using carbon granules (CG) or multi-wall carbon nanotubes deposited on reticulated vitreous carbon (MWCNT-RVC) as electrode materials. For ~ 2 months, the MECs were operated at sulfate loading rates of 21 to 309 gSO₄ -S/m²/d. Although MWCNT-RVC achieved a current density of 57 ± 11 A/m², greater than the 32 ± 9 A/m² observed using CG, both materials exhibited similar sulfate reduction rates (SRR), with MWCNT-RVC reaching 104 ± 16 gSO₄ -S/m²/d while 110 ± 13 gSO₄ -S/m²/d were achieved with CG. Pyrosequencing analysis of the 16S rRNA at the end of experimentation revealed a core community dominated by Desulfovibrio (28%), Methanobacterium (19%) and Desulfomicrobium (14%), on the MWCNT-RVC electrodes. While a similar Desulfovibrio relative abundance of 29% was found in CG-biofilms, Desulfomicrobium was found to be significantly less abundant (4%) and Methanobacterium practically absent (0.2%) on CG electrodes. Surprisingly, our results show that CG can achieve higher current-to-sulfide efficiencies at lower power consumption than the nano-modified three-dimensional MWCNT-RVC.